E-GEOD-51861 - RNA-seq (Illumina and PacBio) of hESC
Released on 30 October 2013, last updated on 25 November 2013
We used PacBio data to identify more reliable transcripts from hESC, based on which we can estimate gene/transcript abundance better from Illumina data. PacBio long reads and Illumina short reads were generated from the same hESC cell line H1. PacBio reads were error-corrected by Illumina reads to identify transcripts. rSeq is used to estimate gene/transcript abundance of the identified transcriptome.
RNA-seq of coding RNA
Kin Fai Au <email@example.com>, Brian A Williams, Eric Schadt, Honoratus Van Bakel, Jason Underwood, Kin-Fai Au, Lawrence Lee, Vittorio Sebastiano, Wing H Wong