E-GEOD-51849 - mRNA expression profiles of antigen-specific CD8+ T cells induced by DNA prime-DNA boost and DNA prime-VACV boost immunization regimen

Released on 30 October 2013, last updated on 3 June 2014
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Similar with others, our data proved that antigen-specific CD8+ T cells from mice primed with DNA and boosted by VACV were much more sensitive to antigen stimulation than those from DNA-boost. Since the mechanisms of in vivo tuning of antigen sensitivity (also termed functional avidity) is still not defined, we compared this two vaccination regimen at gene expression level. Results provide important information of which genes were selectively activated by VACV boost vaccination. For example, data shows that the expression levels of genes involved in Cancer and Wnt signaling pathways is more higher in DNA prime-VACV boost regimen that DNA prime-DNA boost vaccination. To obtain sufficient of antigen-specific cells for microarray analysis, the OVA-specific CD8+ T cells from OT-1 mice were adoptively transferred into wild type mice and then immunized by DNA and VACV vaccine encoding OVA. Four week later, mice were scarificed and antigen-specific CD8+ T cells were emriched by CD45.1-PE antibody and anti-PE MicroBeads from splenocytes.Total RNA was extracted by the RNeasy Mini Kit (QIAGEN, Germany). Followed by amplification and biotin labeling, the samples were hybridized using Illumina Total Prep RNA Amplification Kit (Ambion, USA). Mouse WG-6v2 Expression BeadChips were used for analysis of transcriptome.
Experiment type
transcription profiling by array 
Hu Zhidong <huzhidong2008@163.com>, Chao Qiu, Jianqing Xu, Zhidong Hu
Investigation descriptionE-GEOD-51849.idf.txt
Sample and data relationshipE-GEOD-51849.sdrf.txt
Processed data (1)E-GEOD-51849.processed.1.zip
Additional data (1)E-GEOD-51849.additional.1.zip
Array designA-MEXP-1175.adf.txt