E-GEOD-51533 - Directed differentiation of placode from human embryonic stem cells
Released on 4 December 2013, last updated on 3 June 2014
Here we compare the transcriptional profile during the differentiation of hESC into neuroepithelial cells (NSB) (Chambers et. al 2009 Nature Biotechnology) with our new placode differentiation (PIP). Total RNA was isolated at day 1,3, 5 (NSB), 5 (PIP), 7 (NSB), 7 (PIP), 9 (NSB), 9 (PIP), 11 (NSB) and 11 (PIP) of differentiation using Trizol. Samples for each group in triplicate were processed for Illumina bead arrays (Illumina HT-12) by the MSKCC genomics core facility according to the specifications of the manufacturer.
transcription profiling by array
Jeffrey Zhao <email@example.com>, Lorenz Studer, Zehra Dincer
Specification of Functional Cranial Placode Derivatives from Human Pluripotent Stem Cells. Dincer Z, Piao J, Niu L, Ganat Y, Kriks S, Zimmer B, Shi SH, Tabar V, Studer L. , Europe PMC 24290755