E-GEOD-51505 - Genome-wide incorporation dynamics reveal distinct categories of turnover for the histone variant H3.3

Released on 23 October 2013, last updated on 25 November 2013
Mus musculus
Samples (45)
Protocols (4)
We developed a system to study the DNA replication-independent turnover nucleosomes containing the histone variant H3.3 in mammalian cells. By measuring the genome-wide incorporation of H3.3 at different time points following epitope-tagged H3.3 expression, we find three categories of H3.3-nucleosome turnover in vivo: rapid turnover, intermediate turnover and, specifically at telomeres, slow turnover. Our data indicate that H3.3-containing nucleosomes at enhancers and promoters undergo a rapid turnover that is associated with active histone modification marks including H3K4me1, H3K4me3, H3K9ac, H3K27ac and the histone variant H2A.Z. The rate of turnover is negatively correlated with H3K27me3 at regulatory regions and with H3K36me3 at gene bodies. Examination of incorporation dynamics of histone variant H3.3
Experiment types
ChIP-seq, RNA-seq of coding RNA 
Wenfei Jin <wenfei.jin@nih.gov>, Alika Maunakea, Daniel C Kraushaar, Keji Zhao, Misook Ha
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-51505.idf.txt
Sample and data relationshipE-GEOD-51505.sdrf.txt
Processed data (31)Click to browse processed data
Additional data (1)E-GEOD-51505.additional.1.zip