E-GEOD-5142 - Transcription profiling of gulahuman CD8 T lyphocytes overexpressing hTERT to investigate regulation of profileration
Submitted on 23 June 2006, released on 13 June 2008, last updated on 27 March 2012
Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD2 was profiled on U133Plus 2.0 and submitted as a separate GEO series. Experiment Overall Design: triplicates of 4 conditions, 1 chip (hTERT_YOUNG_HD1_REP2) excluded because of low quality hybridization.
transcription profiling by array, unknown experiment type
Mechanisms regulating the proliferative potential of human CD8+ T lymphocytes overexpressing telomerase. Olivier Menzel, Marco Migliaccio, Darlene R Goldstein, Sophie Dahoun, Mauro Delorenzi, Nathalie Rufer. J Immunol 177(6):3657-68 (2006)