E-GEOD-51375 - Assesing an improved protocol for plasma microRNA extraction

Status
Released on 18 October 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (14)
Array (1)
Protocols (5)
Description
The first step in biomarkers discovery is to identify the best protocols for their purification and analysis. We have identified an optimal RNA extraction method of microRNAs from human plasma samples. We also report that the addition of low doses of carrier RNA before starting RNA extraction improves microRNA extraction and quantification. Human plasma and matched biopsies were obtained from healthy donors and patients attended at the Hospital Universitari i Politècnic La Fe (Valencia, Spain). RNA was extracted by different preanalytal conditions and reagents, testing the suitable of carrier addition at differnt doses. The best protocol was followed up by hybridation on Affymetrix microarrays.
Experiment type
transcription profiling by array 
Contacts
Dolors Sanchez-Izquierdo <arrays_service@iislafe.es>, Dolors Sánchez-Izquierdo, Inés Moret-Tatay
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-51375.idf.txt
Sample and data relationshipE-GEOD-51375.sdrf.txt
Raw data (1)E-GEOD-51375.raw.1.zip
Processed data (1)E-GEOD-51375.processed.1.zip
Additional data (1)E-GEOD-51375.additional.1.zip
Array designA-GEOD-16384.adf.txt
Links