normalization data transformation protocol
Data were extracted using Feature Extraction software (v10.5.1, Agilent) and Gene-Spring GX software (v11.0.1, Agilent). ID_REF = VALUE = quantile normalized log2 signal
array scanning protocol
Microarray results were extracted using Agilent G2565BA Scanner and Feature Extraction software (v10.5.1, Agilent), and subsequently analyzed by Gene-Spring GX software (v11.0.1, Agilent).
Cy3-labeled cRNA (800ng each) were hybridized to the zebrafish microarray (G5219F, Agilent) for 17 h at 65°C.
Purified total RNA of each sample was amplified and labeled with a fluorescent dye Cyanine 3 (Cy3) using a low-RNA input linear amplification kit following the manufacturer's protocol (5184-3523, Agilent).
Adult zebrafish (6 months old, male) were maintained at 28°C under 14h: 10h light/dark cycle.
nucleic acid extraction protocol
Total RNA of each brain was extracted using Trizol (Invitrogen) according to the manufacturer’s instruction. The quantity and quality of the RNA samples were assessed with a NanoDrop ND-1000 spectrometer (NanoDrop Technologies) and an Agilent 2100 bioanalyzer (Agilent).
sample treatment protocol
Every fish was killed by rapid decapitation, and the whole brain was removed and frozen immediately in liquid nitrogen and stored in -80°C.