E-GEOD-5126 - Transcription profiling of mouse Hoxb1 mutant mRNA levels in rhombomere 4 at E10.5

Status
Submitted on 22 June 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (2)
Description
Hoxb1 is required for proper specification of rhombomere 4 and the facial motor neurons. This study analyzed gene expression in the corresponding hindbrain segment of E10.5 mutant embryos. Several genetic pathways were found altered, including transcription factors such as Phox2b, Gata3, Nkx2-2 and Nkx6-1. Experiment Overall Design: Each of the three mutant and control samples was an independent biological replicate. Pools of r4 segments from multiple embryos were snap frozen on dry ice and stored at -80ºC. Total RNA was isolated, processed with standard Affymetrix protocols and hybridized to GeneChip Mouse Expression Sets 430A.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Reversal of Hox1 gene subfunctionalization in the mouse. Petr Tvrdik, Mario R Capecchi. Dev Cell 11(2):239-50 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5126.idf.txt
Sample and data relationshipE-GEOD-5126.sdrf.txt
Raw data (1)E-GEOD-5126.raw.1.zip
Processed data (1)E-GEOD-5126.processed.1.zip
Array designA-AFFY-36.adf.txt
R ExpressionSetE-GEOD-5126.eSet.r
Links