8 protocols
normalization data transformation protocol
GCRMA (using Bioconductor gcrma package) ID_REF = VALUE = Normalized signal (log2 scale)
array scanning protocol
Arrays were scanned using the Affymetrix GeneArray Scanner
hybridization protocol
Affymetrix Generic Hybridization
labelling protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
sample treatment protocol
DMEM, 0.5%BCS, 2 mM glutamine, 0 to 15 ng/ml TGFbeta
nucleic acid extraction protocol
Trizol extraction of total RNA was performed according to the manufacturer's instructions
growth protocol
DMEM, 10%BCS, 2 mM glutamine