7 protocols
normalization data transformation protocol
The data were normalised using quantile normalisation with IlluminaGUI in R ID_REF = VALUE = quantile normalized Detection Pval =
array scanning protocol
Standard Illumina scanning protocol
hybridization protocol
Standard Illumina hybridization protocol
labelling protocol
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
growth protocol
Cells were cultured in DMEM supplemented with 5% FBS, 100 U/ml penicillin, and 100 g/ml streptomycin. Lentiviral vector transfected cells were selected by puromycin treatment.
sample treatment protocol
Cells were treated with 3 IU/ml of IFN beta for 6 hrs. Cells were transfected with empty lentiviral vector or vector containing Y701F STAT1 cDNA.
nucleic acid extraction protocol
Total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Nanodrop.