E-GEOD-50932 - Analysis of gene expression with the Hmga2 allelic series

Status
Released on 18 September 2013, last updated on 25 November 2013
Organism
Mus musculus
Samples (18)
Protocols (2)
Description
In this study, we introduced a series of expression constructs into a mouse lung cancer cell line (482N1): (1) a control short hairpin RNA (shluc) plus the empty expression vector (empty); (2) an shRNA targeting Hmga2 (shHmga2) plus empty; (3) shHmga2 plus an expression vector for the full-length Hmga2 cDNA with the shRNA site mutated (shHmga2 wt); (4) shHmga2 plus the full-length shRNA-mutated Hmga2 with let-7 sites in the 3' UTR mutated (shHmga2 m7); (5) shHmga2 plus the full-length shRNA-mutated Hmga2 with the start codon mutated (shHmga2 ATG wt); and (6) shHmga2 plus the full-length shRNA-mutated Hmga2 with the start codon and let-7 sites mutated (shHmga2 ATG m7). Cells were plated in triplicate, RNA was prepared using RNA-Bee as per manufacturers' instructions and RNA-seq studies were performed using standard methods. For further details on library construction and next-generation sequencing, please contact the contributor.
Experiment type
RNA-seq of coding RNA 
Contacts
Julian Downward <julian.downward@cancer.org.uk>, Elena Armenteros-Monterroso, Madhu S Kumar, Monte M Winslow, Nik Matthews, Philip East, Probir Chakravorty
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-50932.idf.txt
Sample and data relationshipE-GEOD-50932.sdrf.txt
Additional data (1)E-GEOD-50932.additional.1.zip
Links