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E-GEOD-50469 - The molecular phenotype of endocapillary proliferation in IgA nephropathy and potential modulation by bioactive small molecules

Status
Released on 18 May 2015, last updated on 24 May 2015
Organism
Homo sapiens
Samples (22)
Arrays (2)
Protocols (5)
Description
Endocapillary proliferation is associated with higher risk of progressive disease in IgAN. To better understand molecular pathways involved in the development of endocapillary proliferation and to identify novel specific therapeutic targets, we evaluated the glomerular transcriptome of microdissected kidney biopsies from 22 patients with IgAN. Endocapillary proliferation was defined according to the Oxford scoring system by 3 nephropathologists. We analyzed mRNA expression using microarrays and identified transcripts differentially expressed in patients with endocapillary proliferation. Next, we employed both transcription factor analysis and in silico drug screening and confirmed that the endocapillary proliferation transcriptome is significantly enriched with pathways modulated by corticosteroid exposure. With this approach we also identified novel therapeutic targets and bioactive small molecules that may be considered for therapeutic trials for treatment of IgAN. RNA from glomerular compartment was extracted and processed for hybridization on Affymetrix microarrays.
Experiment type
transcription profiling by array 
Contacts
Celine C Berthier, Elisabeth Grone, Heather N Reich, Hermann-Josef Grone, Jeff Hodgin, Matthias Kretzler, Rohan John, Stefan Porubsky
Citation
The molecular phenotype of endocapillary proliferation: novel therapeutic targets for IgA nephropathy. Hodgin JB, Berthier CC, John R, Grone E, Porubsky S, Gr�ne HJ, Herzenberg AM, Scholey JW, Hladunewich M, Cattran DC, Kretzler M, Reich HN.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-50469.idf.txt
Sample and data relationshipE-GEOD-50469.sdrf.txt
Raw data (1)E-GEOD-50469.raw.1.zip
Processed data (1)E-GEOD-50469.processed.1.zip
Additional data (1)E-GEOD-50469.additional.1.zip
Array designsA-GEOD-16356.adf.txt, A-GEOD-17662.adf.txt
Links