Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-49817 - IL-2-dependent gene expression by human regulatory T cells
Released on 9 July 2015, last updated on 19 August 2015
This study determined the genes that are differetially expressed when regulatory T cells were stimulated in vitro with IL-2 388 Affymetrix targets were found to be up or down regulated in human regulatory T cells by IL-2 by two-fold or more. Gene enrichment analyss identified that IL-2 functions to regulate gene expression for mRNAs important for immune system processes, cell growth, cell death, inflammatory response, cell migration, and the Jak/Stat pathway, among others. A subset of these differentially expressed genes were distinctively expressed between individual subjects. Human regulatory T cells (Tregs) cells were FACS purified based on expression of CD4+ CD127-lo CD25-HI. These cells were cultured for 24 hr in media with or without IL-2 (100 u/ml). The cells were harvested, total RNA was prepared and processed to probe Affymetrix human Gene ST1.0 arrays.
transcription profiling by array
Thomas R Malek <email@example.com>, Alberto Pugliese
Selective IL-2 Responsiveness of Regulatory T Cells Through Multiple Intrinsic Mechanisms Supports the Use of Low-Dose IL-2 Therapy in Type 1 Diabetes. Yu A, Snowhite I, Vendrame F, Rosenzwajg M, Klatzmann D, Pugliese A, Malek TR.