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E-GEOD-49817 - IL-2-dependent gene expression by human regulatory T cells

Status
Released on 9 July 2015, last updated on 19 August 2015
Organism
Homo sapiens
Samples (18)
Array (1)
Protocols (6)
Description
This study determined the genes that are differetially expressed when regulatory T cells were stimulated in vitro with IL-2 388 Affymetrix targets were found to be up or down regulated in human regulatory T cells by IL-2 by two-fold or more. Gene enrichment analyss identified that IL-2 functions to regulate gene expression for mRNAs important for immune system processes, cell growth, cell death, inflammatory response, cell migration, and the Jak/Stat pathway, among others. A subset of these differentially expressed genes were distinctively expressed between individual subjects. Human regulatory T cells (Tregs) cells were FACS purified based on expression of CD4+ CD127-lo CD25-HI. These cells were cultured for 24 hr in media with or without IL-2 (100 u/ml). The cells were harvested, total RNA was prepared and processed to probe Affymetrix human Gene ST1.0 arrays.
Experiment type
transcription profiling by array 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-49817.idf.txt
Sample and data relationshipE-GEOD-49817.sdrf.txt
Raw data (1)E-GEOD-49817.raw.1.zip
Processed data (1)E-GEOD-49817.processed.1.zip
Array designA-AFFY-141.adf.txt
Links