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E-GEOD-49436 - the analysis of piRNA shows the function of Slicer activity of AGO3 in piRNA biogenesis

Status
Released on 30 June 2014, last updated on 14 July 2014
Organism
Drosophila melanogaster
Samples (3)
Protocols (2)
Description
Piwi-interacting RNAs (piRNAs) silence transposons in animal germ cells. In Drosophila, the reciprocal “Ping-Pong” cycle of piRNA-directed RNA cleavage, catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (AGO3) through their Slicer activity, is believed to expand the population of antisense piRNAs in response to transposon expression. Whether and how the Slicer activity of AGO3/Aub promotes the process of the secondary piRNA amplification remain unclear. Here we generated transgenic flies that could express AGO3 Slicer mutant forms to ellucidate the Slicer activity of AGO3. small-RNA libraries from 3 samples of D. mel. Ovaries.
Experiment type
RNA-seq of non coding RNA 
Contacts
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-49436.idf.txt
Sample and data relationshipE-GEOD-49436.sdrf.txt
Processed data (1)E-GEOD-49436.processed.1.zip
Links