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E-GEOD-49298 - Identification of microRNAs differentially expressed in prostatic secretions of patients with prostate cancer

Status
Released on 27 September 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (5)
Description
In this study, we aimed to identify a miRNA expression signature that could be used to distinguish PCa from BPH. We have shown for the first time in the literature the presence of miRNAs in the PSS. We suggest PSS as a powerful non-invasive source for evaluation of prognosis in PCa, since prostate massages can be easily applied during routine examination. Our results showed that certain differentially expressed miRNAs in PSS could be used as diagnostics markers. Prostate secretion samples (PSS) from 23 PCa and 25 benign prostate hyperplasia (BPH) patients were obtained from Urology Department of Bagcilar Educational and Research Hospital (Istanbul). MicroRNA (miRNA) profiling of eight PSS (four from BPH, four from PCa patients) were performed using microarray. Four of significantly deregulated miRNAs were further confirmed using quantitative reverse-transcription PCR (qRT-PCR). Statistical analysis was performed using Student’s t-test. ROC curves were plotted with SPSS-15.0.
Experiment type
transcription profiling by array 
Contacts
Atilla Semercioz, Chad J Creighton, Esra Guzel, Michael Mittmann, Mustafa Ozen, Omer F Karatas, Serdar Aykan, Serhat Yentur, Sinan Ekici
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-49298.idf.txt
Sample and data relationshipE-GEOD-49298.sdrf.txt
Raw data (1)E-GEOD-49298.raw.1.zip
Processed data (1)E-GEOD-49298.processed.1.zip
Array designA-GEOD-15018.adf.txt
Links