E-GEOD-49218 - Gene regulation by CBP-p300 in the lens placode - E10.5
Released on 23 September 2013, last updated on 3 June 2014
Wild type or CBP/p300 fx/fx; Pax6-Cre-positive (CBP/p300-/-) surface ectoderm from E10.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays. Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.
transcription profiling by array
David Cy Beebe <firstname.lastname@example.org>, David C Beebe, Jie Huang, Ying Liu
Histone posttranslational modifications and cell fate determination: lens induction requires the lysine acetyltransferases CBP and p300. Wolf L, Harrison W, Huang J, Xie Q, Xiao N, Sun J, Kong L, Lachke SA, Kuracha MR, Govindarajan V, Brindle PK, Ashery-Padan R, Beebe DC, Overbeek PA, Cvekl A. , Europe PMC 24038357