E-GEOD-49152 - miRNA expression in human chondrocyte subtypes
Released on 23 October 2013, last updated on 11 June 2014
Frozen blocks of human embryonic limb tissue were sectioned and laser capture microdissected to collect three human chodrocyte tissues - precursor, differentiated and hypertrophic chondrocytes.Total RNA including miRNAs was isolated and analyzed using Applied Biosystems OpenArray® Real-Time PCR system. RT-PCR miRNA expression profiling. miRNA expression from three chondrocyte sub types - precursor, differentiated and hypertrophic chondrocytes were analyzed using TaqMan® OpenArray® Human MicroRNA Panel.
transcription profiling by RT-PCR
Nobish Varghese <firstname.lastname@example.org>, Audrey McAlinden