E-GEOD-48997 - Expression data in wt or mutant Drosophila melanogaster embryos

Status
Released on 20 July 2013, last updated on 15 July 2015
Organism
Drosophila melanogaster
Samples (15)
Array (1)
Protocols (7)
Description
Terminal differentiation of epidermal cells in Drosophila embryos requires the activity of a transcription factor. Svb is necessary and sufficient to induce this process. pri is a regulator of Svb activity, converting it from a repressor into an activator. To characterize the downstream Svb and pri effectors in cell morphogenesis, we performed microarrays in wt, svb -/- (no gene) and pri -/- (svb repressor) mutant conditions. Embryos were selected at a precise 13-15h (after egg laying) stage of development, and manually genotyped for each condition: wt (W samples), svb -/- (R samples) and pri -/- (P samples). 5 independent replicates of 200 embryos are used for each condition. RNA were extracted and hybridized on Affymetrix microarrays.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-48997.idf.txt
Sample and data relationshipE-GEOD-48997.sdrf.txt
Raw data (1)E-GEOD-48997.raw.1.zip
Processed data (1)E-GEOD-48997.processed.1.zip
Additional data (1)E-GEOD-48997.additional.1.zip
Array designA-AFFY-35.adf.txt
Links