E-GEOD-48989 - Cyclin D1 determines estrogen dependent signaling in human breast cancer cell line MCF7

Released on 18 July 2013, last updated on 29 July 2013
Homo sapiens
Samples (8)
Array (1)
Protocols (7)
The CCND1 gene, which is frequently overexpressed in cancers, encodes the regulatory subunit of a holoenzyme that phosphorylates the retinoblastoma protein (pRb). It is known that cyclin D1 regulates ERα transactivation using heterologous reporter systems, the significance of this observation to E2 dependent gene activation is unknow. E2 stimulated MCF7 cells treated with cyclin D1 siRNA in order to analyze the genes regulated by estradiol in a cyclin D1 dependent manner. Hormone deprived MCF7 cells were treated with cyclin D1 siRNA or control siRNA and stimulated with E2 or vehicle Four separate 10cm plates of MCF7 cells treated with control siRNA were compared to four 10cm plates of MCF7 cells treated with cyclin D1 siRNA. 2 plates in each group treated with vehicle and two plates treated with E2.
Experiment type
transcription profiling by array 
Mathew Casimiro Casimiro <geo@ncbi.nlm.nih.gov>, Mathew C Casimiro, Richard G Pestell
Investigation descriptionE-GEOD-48989.idf.txt
Sample and data relationshipE-GEOD-48989.sdrf.txt
Raw data (1)E-GEOD-48989.raw.1.zip
Processed data (1)E-GEOD-48989.processed.1.zip
Array designA-AFFY-141.adf.txt