E-GEOD-48962 - Transcription profiling by high throughput sequencing in wild type and R6/2 mutant mouse cortex and striatum at 8 and 12 weeks

Status
Released on 18 July 2013, last updated on 12 May 2016
Organism
Mus musculus
Samples (24)
Protocols (2)
Description
Transcriptional dysregulation is an early feature of Huntington's disease (HD). We observed gene-specific changes in H3K4me3 at transcriptionally repressed promoters in R6/2 mouse and human HD brain. Genome-wide analysis showed a novel chromatin signature for this mark. Reducing the levels of the H3K4 demethylase SMCX/Jarid1c in primary neurons reversed down-regulation of key neuronal genes caused by mutant Huntingtin (Htt) expression. Finally, reduction of SMCX/Jarid1c in primary neurons from BACHD mice or the single Jarid1 in a Drosophila HD model was protective. Therefore, targeting this epigenetic signature may be an effective strategy to ameliorate the consequences of HD. mRNA-seq in wild type and R6/2 cortex and striatum at 8 and 12 weeks.
Experiment type
RNA-seq of coding RNA 
Contacts
Christopher Ng <geo@ncbi.nlm.nih.gov>, Christopher W Ng, David Housman, Ernest Fraenkel, Leslie M Thompson, Malini Vashishtha, Theresa Gipson
Citation
Targeting H3K4 trimethylation in Huntington disease. Vashishtha M, Ng CW, Yildirim F, Gipson TA, Kratter IH, Bodai L, Song W, Lau A, Labadorf A, Vogel-Ciernia A, Troncosco J, Ross CA, Bates GP, Krainc D, Sadri-Vakili G, Finkbeiner S, Marsh JL, Housman DE, Fraenkel E, Thompson LM. , Europe PMC 23872847
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-48962.idf.txt
Sample and data relationshipE-GEOD-48962.sdrf.txt
Processed data (1)E-GEOD-48962.processed.1.zip
Additional data (1)E-GEOD-48962.additional.1.zip
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