normalization data transformation protocol
The data were normalised using quantile normalisation with preprocessCore in R. ID_REF = VALUE = quantile normalized signal intensity
array scanning protocol
Standard Illumina scanning protocol.
Standard Illumina hybridization protocol.
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
nucleic acid extraction protocol
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.