E-GEOD-48864 - Expression analysis of Aspergillus oryzae grown on different carbon sources

Status
Released on 15 July 2013, last updated on 3 June 2014
Organism
Aspergillus oryzae RIB40
Samples (32)
Array (1)
Protocols (6)
Description
In this study, we focused on chemically defined inducers or substrates to drive expression of cellulases, hemicellulases and accessory enzymes in the model filamentous fungus Aspergillus oryzae. Cellohexaose (O-CHE), mannohexaose (O-MHE), xylopentaose (O-XPE), arabinoheptaose (O-AHP), 1,3:1,4-β-glucohexaose (O-BGHEXA), 63-α-D-glucosyl-maltotriosyl-maltotriose (O-GMH), 61-α-D-galactosyl-mannotriose (O-GM3), xyloglucan (X3Glc4-borohydride reduced; O-X3G4R), turanose (TYR) and sophorose (SOP) were used to induce the plant polysaccharide degradation machinery of A. oryzae. The strain used in this study was the A. oryzae sequenced strain RIB40, obtained from IBT culture collection at Technical University of Denmark. To obtain a global view of the A. oryzae transcriptome activated for plant biomass conversion, mRNA from growth after 2 h on 10 different carbohydrate active enzyme inducers (di- and –oligo saccharides) was subjected to custom-designed Agilent microarray analysis.
Experiment type
transcription profiling by array 
Contacts
D.B.R.K. Gupta Udatha <gupta.udatha@outlook.com>, D G Udatha, Evangelos Topakas, John A Smith, Lisbeth Olsson, Margarita Salazar, Melanie Z Khodaie, Mikael R Andersen
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-48864.idf.txt
Sample and data relationshipE-GEOD-48864.sdrf.txt
Raw data (1)E-GEOD-48864.raw.1.zip
Processed data (1)E-GEOD-48864.processed.1.zip
Additional data (1)E-GEOD-48864.additional.1.zip
Array designA-GEOD-17444.adf.txt
Links