Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-4885 - shRNA profiling of human Hep2G cells knocked down for SRC-1 expressio to investigate the role of coactivator SRC-1/NcoA-1 for IL-6 target gene induction
Submitted on 22 May 2006, released on 13 June 2008, last updated on 10 June 2011
This experiment series addresses the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. For that purpose, HepG2 human hepatocellular carcinoma cells were manipulated to stably express an shRNA that knocks down SRC-1 expression yielding the HepG2-∆Src1 cells. Either unmanipulated HepG2 or HepG2-∆Src1 cells were then treated for various periods with IL-6. Experiment Overall Design: HepG2 or HepG2-∆Src1 (with silenced SRC-1/NcoA-1 expression) cells were either left untreated or treated with 10 ng/ml IL-6 for 1 or 4 hours. Every experiment was carried out in replicate.
RNAi profiling by array, unknown experiment type