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E-GEOD-4885 - shRNA profiling of human Hep2G cells knocked down for SRC-1 expressio to investigate the role of coactivator SRC-1/NcoA-1 for IL-6 target gene induction

Status
Submitted on 22 May 2006, released on 13 June 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (9)
Description
This experiment series addresses the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. For that purpose, HepG2 human hepatocellular carcinoma cells were manipulated to stably express an shRNA that knocks down SRC-1 expression yielding the HepG2-∆Src1 cells. Either unmanipulated HepG2 or HepG2-∆Src1 cells were then treated for various periods with IL-6. Experiment Overall Design: HepG2 or HepG2-∆Src1 (with silenced SRC-1/NcoA-1 expression) cells were either left untreated or treated with 10 ng/ml IL-6 for 1 or 4 hours. Every experiment was carried out in replicate.
Experiment types
RNAi profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4885.idf.txt
Sample and data relationshipE-GEOD-4885.sdrf.txt
Raw data (1)E-GEOD-4885.raw.1.zip
Processed data (1)E-GEOD-4885.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-4885.eSet.r
Links