Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-48613 - Genome-wide analysis of keratinocyte gene expression during their course of differentiation in presence or absence of JNK1,2-deficient or wildtype immortalized mouse embryonic fibroblasts (MEFs)

Status
Released on 26 November 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (24)
Arrays (2)
Protocols (7)
Description
Analysis of keratinocyte differentiation in presence or absence of fibroblast-derived JNK-dependent soluble factors in vitro. The hypothesis tested in the present study was that JNK-dependent fibroblast-derived soluble factors promote an efficient keratinocyte differentiation. Results provide important information about reduced expression of a subset of differentiation associated genes in keratinocytes in the absence of JNK-dependent fibroblast-derived soluble factors. In vitro transwell co-culture experiments were performed using jnk1-/-jnk2-/- or wildtype immortalized mouse embryonic fibroblasts (MEFs) and differentiating primary normal human epidermal keratinocytes (NHEK) over a time course of 6 days. Total RNA was obtained from NHEK prior to cultivation with MEFs (day 0) and every second day (days 2, 4, and 6) during co-culture. MEFs have been described previously in Sabapathy et al. (2004) Mol Cell 15:713-25.
Experiment type
transcription profiling by array 
Contacts
Peter Angel <p.angel@dkfz-heidelberg.de>, Bettina Füssel, Marion Schumacher, Zbigniew Rogon
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-48613.idf.txt
Sample and data relationshipE-GEOD-48613.sdrf.txt
Processed data (1)E-GEOD-48613.processed.1.zip
Additional data (1)E-GEOD-48613.additional.1.zip
Array designsA-MEXP-1171.adf.txt, A-MEXP-1173.adf.txt
Links