E-GEOD-48206 - Gene Expression Profile for knocking down Tet1 in pre-iPSCs culturing in Vc medium

Released on 27 October 2013, last updated on 25 November 2013
Mus musculus
Samples (6)
Protocols (2)
Tet1 is a hydroxylase known for its role in the conversion of 5-methylcytosines (5mC) to 5-hydroxymethylcytosines (5hmC) involved in the possible active demethylation process and gene expression regulation. As somatic cell reprogramming involves the re-activation of pluripotency genes and the silencing of somatic ones, it remains unclear the role of Tet1 in the reprogramming process. Here, we performed RNAseq for WT and knocking down Tet1 in two pre-iPSCs cell lines, pre2-2 and pre3. Using two pre-iPSCs cell lines: pre2-2 and pre3, cultured by mES medium(+lif), transfected siCtrl/siTet1 siRNA or non-treatment, then, after 16h, the medium were replaced by mES(+lif) medium plus Vc(50ug/ml), total RNA was extracted after another 32h later for RNAseq.
Experiment type
RNA-seq of coding RNA 
Xiaoshan Wang <wang_xiaoshan@gibh.ac.cn>, Duanqing Pei, Guo-Liang Xu
Vitamin C modulates TET1 function during somatic cell reprogramming. Chen J, Guo L, Zhang L, Wu H, Yang J, Liu H, Wang X, Hu X, Gu T, Zhou Z, Liu J, Liu J, Wu H, Mao SQ, Mo K, Li Y, Lai K, Qi J, Yao H, Pan G, Xu GL, Pei D. , Europe PMC 24162740
Exp. designProtocolsVariablesProcessedSeq. reads