5 protocols
AccessionType
normalization data transformation protocol
Missing intensities were removed, raw intensites were quantile normalized and log2 transformed. Poorly variable probe sets were removed by utilising their inter-quantile range (IQR), cut-off value of 0.7 . ID_REF = VALUE = quantile normalized, and log2 transformed signals that passed the stringent QC as described in the data processing section
array scanning protocol
Standard Illumina scanning protocol, using iScan.
hybridization protocol
RNA was hybridized to the Illumina Whole-Genome Expression Beadchips (HT12-v4), using the standard Illumina Hybridization Protocol.
labelling protocol
The isolated RNAs were amplified and labelled using the Illumina TotalPrep RNA Amplification kit (Ambion)
nucleic acid extraction protocol
The isolated RNAs were amplified and labelled using the Illumina TotalPrep RNA Amplification kit (Ambion)