E-GEOD-47643 - Gene expression of in vitro cultivated preB cells before and after 8, 16 and 24 hours induction of miR-221
Released on 5 June 2013, last updated on 3 June 2014
PreB cells were analyzed for differences in gene expression before and after the overexpression of miR-221. In order to dissect possible targets for the miR-221, gene expression profiles of preB cells un-induced or induced for the miR-221 expression after 8, 16 and 24 hours were compared. All induction time-points, e.g. after 8, 16 and 24 hours were compared to un-induced preB cells and to each other group. Gene expression profiles of un-induced preB cells, preB cells induced for miR-221 expression after 8, 16, and 24 hours were analyzed using Affymetrix MG 430 2.0 whole genome arrays. Each time-point was performed in triplicates for un-induced preB cells and preB cells induced for miR-221 expression after 8, 16, and 24 hours (12 arrays in total). To obtain genes significantly downregulated upon induction of miR-221, the expression profiles of un-induced preB cells were compared to preB cells activated for 8, 16, or 24 h and were also compared to each other. After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 µg cRNA hybridized in triplicates for each of the four groups to the 12 GeneChip arrays: Group1, un-induced preB cells, Group2, preB cells induced for miR-221 expression after 8h, Group3, preB cells induced for miR-221 expression after 16h, Group4, preB cells induced for miR-221 expression after 24h.
transcription profiling by array
Joachim R. Grün <Gruen@DRFZ.de>, Corinne Bouquet, Fritz Melchers, Joachim R Grün, Marko Knoll, Szandor Simmons
miR-221 redirects precursor B cells to the bone marrow and regulates their residence. Knoll M, Simmons S, Bouquet C, Grï¿½n JR, Melchers F. , Europe PMC 23716169
SiPaGene: A new repository for instant online retrieval, sharing and meta-analyses of GeneChip expression data. Menssen A, Edinger G, Grï¿½n JR, Haase U, Baumgrass R, Grï¿½tzkau A, Radbruch A, Burmester GR, Hï¿½upl T. , Europe PMC 19265543