E-GEOD-47203 - Genome-wide expression profiling of Hic-5 knockdown in PshTert-AR human myofibroblasts

Status
Released on 26 August 2013, last updated on 2 September 2013
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
Identifying the effect of the co-regulator Hic-5 (TGFB1I1) on global androgen receptor transcriptional activity in PShTert-AR prostate fibroblast cells with view to further elucidating the broader biological role of Hic-5 on fibroblast spceific androgen signaling. Knockdown of Hic5 for 48 hours in PShTert-AR cells significantly altered the androgen regulation of approximately 1347 genes. The effect of Hic-5 knockdown was to suppress androgen regulation of approximately 85% of those transcripts, by either reducing androgen mediated repression or activition of gene expression. PShTert-AR cells were transfected with 10nM non-specific control siRNA (NS) or with commercially avaliable Hic-5 specific siRNA (siHic5) for 24hrs. Cells were kept in 5% hormone stripped FBS RPMI for a further 24 hours and subsequently treated with either ethanol vehicle control or 10nM DHT for 16hr. Total RNA was extracted. Nine independent vehicle and 9 DHT siNS and siHic5 samples were pooled into three groups of vechicle and DHT treated siNS and siHic5 samples and hybrydised to Affymetrix Human Gene 1.0 ST array chips.
Experiment type
transcription profiling by array 
Contacts
damien leach <damien.leach@adelaide.edu.au>, Damien A Leach, Grant Buchanan
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-47203.idf.txt
Sample and data relationshipE-GEOD-47203.sdrf.txt
Raw data (1)E-GEOD-47203.raw.1.zip
Processed data (1)E-GEOD-47203.processed.1.zip
Array designA-AFFY-141.adf.txt
Links