Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-47201 - H. salinarum TrmB dynamics

Status
Released on 7 August 2013, last updated on 12 August 2013
Organism
Halobacterium sp. NRC-1
Samples (48)
Array (1)
Protocols (7)
Description
Previous work characterized TrmB as a global glucose responsive metabolic transcription factor in archaeal extremophiles. However, it remains unclear how TrmB dynamically regulates its ~100 metabolic enzyme-coding genes. Using a dynamic perturbation approach, we elucidate the topology of the metabolic GRN in Halobacterium salinarum. We assayed gene expression in a wild-type and trmB knockout strain before and immedeatly following glucose perturbation. Clustering dynamic gene expression patterns reveals that TrmB functions alone to regulate central metabolic enzyme-coding genes, but cooperates with various regulators to control peripheral metabolic pathways. Gene expression of 100 genes was assayed in two strains - a wild-type and a trmB knockout. Two biological replicates of each strain were performed. Gene expression was assayed 240, 60, and 0 minutes prior to glucose addition and 5,10,20,45,90,180,360 minutes after.
Experiment type
other 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-47201.idf.txt
Sample and data relationshipE-GEOD-47201.sdrf.txt
Processed data (1)E-GEOD-47201.processed.1.zip
Additional data (1)E-GEOD-47201.additional.1.zip
Array designA-GEOD-17188.adf.txt
Links