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E-GEOD-47008 - Solanum torvum transcriptional responses to nematodes

Status
Released on 1 November 2013, last updated on 13 May 2014
Organism
Solanum melongena, Solanum torvum
Samples (12)
Array (1)
Protocols (6)
Description
Solanum torvum Sw is worldwide employed as rootstock for eggplant cultivation because of its vigour and resistance/tolerance to the most serious soil-borne diseasesas bacterial, fungal wilts and root-knot nematodes. A 30,0000 features custom combimatrix chip was designed and microarray hybridizations were conducted for both control and 14 dpi (day post inoculation) with Meloidogyne incognita-infected roots samples. We also tested the chip with samples from the phylogenetically-related nematode-susceptible eggplant species Solanum melongena.The genes identified from S. torvum catalogue, bearing high homology to knownnematode resistance genes, were further investigated in view of their potential role in the nematode resistance mechanism. total RNA was extracted from control and 14 days post-infection (infection with root-knot nematode Meloidogyne incognita) from roots of Solanum torvum and Solanum melongena. Three biological replicates were used for each condition and genotype for a total of 12 samples.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-47008.idf.txt
Sample and data relationshipE-GEOD-47008.sdrf.txt
Raw data (1)E-GEOD-47008.raw.1.zip
Processed data (1)E-GEOD-47008.processed.1.zip
Array designA-GEOD-17170.adf.txt
Links