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E-GEOD-4698 - Transcription profiling of human ALL samples from very early relapsed childhood patients

Status
Submitted on 21 April 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (60)
Array (1)
Protocols (2)
Description
Purpose: In childhood acute lymphoblastic leukemia (ALL), approximately 25% of patients suffer from relapse. In recurrent disease, despite intensified therapy, overall cure rates of 40% remain unsatisfactory and survival rates are particularly poor in certain subgroups. The probability of long-term survival after relapse is predicted from well-established prognostic factors, i. e. time and site of relapse, immunophenotype and minimal residual disease. However, the underlying biological determinants of these prognostic factors remain poorly understood. Results: We show here that patients with very early relapse of ALL are characterized by a distinctive gene expression pattern. We identified a set of 83 genes differentially expressed in very early relapsed ALL compared to late relapsed disease. The vast majority of genes was up-regulated and many were late cell cycle genes with a function in mitosis. In addition, samples from patients with very early relapse showed a significant increase in the percentage of S and G/2M phase cells and this correlated well with the expression level of cell cycle genes. Conclusions: Very early relapse of ALL is characterized by an increased proliferative capacity of leukemic blasts and up-regulated mitotic genes. The latter suggests that novel drugs, targeting late cell cycle proteins, might be beneficial for these patients that typically face a dismal prognosis. Experiment Overall Design: We performed gene expression profiling on 60 prospectively collected samples of children with first relapse of acute lymphoblastic leukemia enrolled on the relapse trial ALL-REZ BFM 2002 of the Berlin-Frankfurt-Muenster study group.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Expression of late cell cycle genes and an increased proliferative capacity characterize very early relapse of childhood acute lymphoblastic leukemia. Renate Kirschner-Schwabe, Claudio Lottaz, Jörn Tödling, Peter Rhein, Leonid Karawajew, Cornelia Eckert, Arend von Stackelberg, Ute Ungethüm, Dennis Kostka, Andreas E Kulozik, Wolf-Dieter Ludwig, Günter Henze, Rainer Spang, Christian Hagemeier, Karl Seeger. Clin Cancer Res 12(15):4553-61 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4698.idf.txt
Sample and data relationshipE-GEOD-4698.sdrf.txt
Raw data (2)E-GEOD-4698.raw.1.zip, E-GEOD-4698.raw.2.zip
Processed data (1)E-GEOD-4698.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-4698.eSet.r
Links