E-GEOD-46911 - Transcripts induced by recombinant IFNa in healthy blood monocytes at different time points
Released on 3 June 2014, last updated on 7 June 2014
To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I IFN (IFN-α2b) in vitro. RNA was extracted at different incubation times (1, 6, 24, 48 and 72 hrs) and the expression data was normalized to that of monocytes cultured with medium. Blood monocytes isolated from healthy volunteers were incubated with 20% autologous serum alone or in the presence of 1000 U/ml of IFNα2b (Schering Plough, Kenilworth, NJ) in 6-well plates at a concentration of 106 monocytes per well in 3 ml of media. After incubation for one hour at 37 ⁰C, cells were harvested and RNA was extracted. Identical experiments were done after the following incubation time points: six hours, twenty four hours, two days, and three days.
transcription profiling by array
Alicia Rodriquez-Pla, Alisa Gotte, Holden Maecker, Jacques Banchereau, Jose Rossello-Urgell, Karolina A Palucka, Lorien Nassi, Lynda Bennett, Marilynn Punaro, Nicole Baldwin, Pinakeen Patel, Victoria Cantrell, Virginia Pascual
IFN Priming Is Necessary but Not Sufficient To Turn on a Migratory Dendritic Cell Program in Lupus Monocytes. Rodriguez-Pla A, Patel P, Maecker HT, Rossello-Urgell J, Baldwin N, Bennett L, Cantrell V, Baisch J, Punaro M, Gotte A, Nassi L, Wright T, Palucka AK, Banchereau J, Pascual V. , Europe PMC 24829414