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E-GEOD-46902 - FANCD2 Activates Transcription of TAp63 and Suppresses Tumorigenesis

Status
Released on 27 June 2013, last updated on 17 July 2013
Organism
Homo sapiens
Samples (2)
Protocols (3)
Description
Fanconi Anemia (FA) is a rare genetic disorder characterized by an increased susceptibility to squamous cell cancers. Fifteen FA genes are known, and the encoded proteins cooperate in a common DNA repair pathway. A critical step is the monoubiquitination of the FANCD2 protein, and cells from most FA patients are deficient in this step. How monoubiquitinated FANCD2 suppresses squamous cell cancers is unknown. Here we show that Fancd2-deficient mice are prone to Ras oncogene-driven skin carcinogenesis, while Usp1-deficient mice, expressing elevated cellular levels of Fancd2-Ub, are resistant to skin tumors. Moreover, Fancd2-Ub activates the transcription of the tumor suppressor TAp63, thereby promoting cellular senescence and blocking skin tumorigenesis. For FA patients, the reduction of FANCD2-Ub and TAp63 protein levels may account for their susceptibility to squamous cell neoplasia. Taken together, Usp1 inhibition may be a useful strategy for upregulating TAp63 and preventing or treating squamous cell cancers in the general non-FA population. Examination of FANCD2 binding after UV treatment in 293T cells
Experiment type
ChIP-seq 
Contacts
eunmi Park <eunmi_park@dfci.harvard.edu>, Alan D D'Andrea, Eunmi Park
Citation
FANCD2 Activates Transcription of TAp63 and Suppresses Tumorigenesis. Park E, Kim H, Kim JM, Primack B, Vidal-Cardenas S, Xu Y, Price BD, Mills AA, D'Andrea AD. , PMID:23806336
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-46902.idf.txt
Sample and data relationshipE-GEOD-46902.sdrf.txt
Processed data (1)E-GEOD-46902.processed.1.zip
Links