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E-GEOD-46861 - PTHR1 knock-down in osteosarcoma cells

Status
Released on 1 July 2015, last updated on 19 August 2015
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
Expression analysis of OS tumors with shRNA knockdown of PTHR1 mouse OS 80 (a Cre:lox OS cell line from Boston) was tagged with firefly luciferase expression construct. They were then infected with either control (renilla luciferase shRNA) or an shRNA that effective knocks-down PTHR1 (PTHR1.358; para-thyroid hormone receptor). The cells were injected with matrigel onto the back flank of Balb/c nu/nu mice and left to grow for 1 month with weekly monitoring of tumour size by in vivo luciferase assay. At 4 weeks the tumours were removed and trizol stored. Whole tumour was ground up and set form micro-array. 6 tumors with shRNA PTHR1 knockdown ; 6 tumors with shRNA control (renilla luciferase shRNA) knockdown
Experiment type
transcription profiling by array 
Contacts
Alistair Morgan Chalk <alistair.chalk@gmail.com>, Alistair Chalk, Ankita Goraida, Carl Walkley, Jack Martin, Megan Russell, Patricia Ho
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46861.idf.txt
Sample and data relationshipE-GEOD-46861.sdrf.txt
Raw data (1)E-GEOD-46861.raw.1.zip
Processed data (1)E-GEOD-46861.processed.1.zip
Array designA-AFFY-130.adf.txt
Links