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E-GEOD-46844 - Expression data of kidney multiciliated cells from zebrafish embryos

Status
Released on 11 May 2013, last updated on 3 June 2014
Organism
Danio rerio
Samples (4)
Array (1)
Protocols (7)
Description
Multiciliated cells (MCCs) possess multiple motile cilia on the cell surface and are widely distributed throughout the vertebrate body to perform important physiological functions by regulating fluid movement in the intercellular space. However, neither their function during organ development nor the molecular mechanisms underlying multiciliogenesis are yet well understood. We aim to study the function of miR-34b in multiciliogenesis. We used microarrays to study the global gene expression change in multiciliated cells after miR-34b knockdown. Multiciliated cells were isolated from 3 dpf control embryos (injected with control morpholino) and miR-34b morphants (injected with miR-34b morpholino) for RNA extraction and hybridization on Affymetrix microarrays. Two independent sets of biological replicates were used.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46844.idf.txt
Sample and data relationshipE-GEOD-46844.sdrf.txt
Raw data (1)E-GEOD-46844.raw.1.zip
Processed data (1)E-GEOD-46844.processed.1.zip
Array designA-AFFY-38.adf.txt
Links