E-GEOD-46839 - ChIP-chip of DSB factors in the presence and absence of S phase checkpoint induction
Released on 18 September 2013, last updated on 3 June 2014
ChIP-chip was used to compare DSB factor localization in wild-type and checkpoint mutant strains under S phase checkpoint inducing and uninducing conditions. We analyzed the DNA binding of factors associated with meiotic DNA replication and DSB formation by chromatin immunoprecipitation (ChIP), including; the replicative helicase Mcm2-7 and the meiotic DSB factors Mer2, Rec114 and Mre11. Immunoprecipitated and input DNA samples were differentially labeled and cohybridized to a single microarray. We have included 2-3 biological replicates of each experiment, including one dye swap experiment.
ChIP-chip by tiling array
Andreas Hochwagen <email@example.com>, Hannah G Blitzblau