E-GEOD-46839 - ChIP-chip of DSB factors in the presence and absence of S phase checkpoint induction

Status
Released on 18 September 2013, last updated on 3 June 2014
Organism
Saccharomyces cerevisiae
Samples (54)
Array (1)
Protocols (6)
Description
ChIP-chip was used to compare DSB factor localization in wild-type and checkpoint mutant strains under S phase checkpoint inducing and uninducing conditions. We analyzed the DNA binding of factors associated with meiotic DNA replication and DSB formation by chromatin immunoprecipitation (ChIP), including; the replicative helicase Mcm2-7 and the meiotic DSB factors Mer2, Rec114 and Mre11. Immunoprecipitated and input DNA samples were differentially labeled and cohybridized to a single microarray. We have included 2-3 biological replicates of each experiment, including one dye swap experiment.
Experiment type
ChIP-chip by tiling array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46839.idf.txt
Sample and data relationshipE-GEOD-46839.sdrf.txt
Processed data (1)E-GEOD-46839.processed.1.zip
Additional data (1)E-GEOD-46839.additional.1.zip
Array designA-GEOD-5991.adf.txt
Links