E-GEOD-4678 - Transcription profiling of two transgenic models familial hypertrophic cardiomyopathy mice vs wild type and non transgenic controls using pooled and non pooled heart extracts to identify differences in gene expression in FHC

Status
Submitted on 17 April 2006, released on 23 November 2007, last updated on 27 March 2012
Organism
Mus musculus
Samples (10)
Array (1)
Protocols (5)
Description
Familial hypertrophic cardiomyopathy (FHC) is a disease characterized by ventricular hypertrophy, fibrosis, and aberrant systolic and/or diastolic function. We previously developed two transgenic mouse models that carry FHC associated mutations in alpha-tropomyosin (TM): FHC alpha-TM175 mice show patchy areas of mild ventricular disorganization and limited hypertrophy; whereas FHC alpha-TM180 mice exhibit severe hypertrophy and fibrosis and die within 6 months. To obtain a better understanding of the molecular mechanisms associated with the early onset of cardiac hypertrophy, we conducted a detailed comparative analysis of gene expression in 2.5-month-old control and FHC alpha-TM175 and alpha-TM180 ventricular tissue. Results show that 754 genes (from a total of 22,600) were differentially expressed between the NTG and the FHC hearts. There are 178 differentially regulated genes between NTG and the FHC alpha-TM175 hearts, 388 genes are differentially expressed between NTG and FHC alpha-TM180 hearts, and 266 genes are differentially expressed between FHC alpha-TM175 and FHC alpha-TM180 hearts. Genes that exhibit the largest increase in expression belong to the secreted/extracellular matrix category, and those with the most significant decrease in expression are associated with metabolic enzymes. Samples used for hybridization consisted of pooled (P) and non-pooled (NP) RNA extracts from the three groups namely NTG, alpha-TM175 and alpha-TM180. Ten hybridization experiments were performed in which each genotypic group was represented by two or more non-pooled (NP) individual RNA extracts and one pooled (P) sample that resulted from combining 20 individual heart extracts
Experiment types
transcription profiling by array, disease state
Contact
Citation
Microarray analysis of gene expression during early stages of mild and severe cardiac hypertrophy. Sudarsan Rajan, Sarah S Williams, Ganapathy Jagatheesan, Rafeeq P H Ahmed, Geraldine Fuller-Bicer, Arnold Schwartz, Bruce J Aronow, David F Wieczorek. Physiol Genomics 27(3):309-17 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4678.idf.txt
Sample and data relationshipE-GEOD-4678.sdrf.txt
Processed data (1)E-GEOD-4678.processed.1.zip
Array designA-AFFY-36.adf.txt
Links