E-GEOD-46664 - Digital Gene Expression Tag Profiling after Wt1 deletion in Sertoli cell

Status
Released on 6 May 2014, last updated on 30 May 2014
Organism
Mus musculus
Samples (2)
Protocols (4)
Description
The Wilms tumor gene, Wt1, is specifically expressed in Sertoli cells (SCs) which support spermatogenesis. Germ cell loss was found in Wt1 knockout mice. In vitro studies demonstrated that Wt1 was essential for cell polarity maintenance in SCs. We used Digital Gene Expression Tag Profiling to detail the global programme of gene expression after Wt1 deletion in sertoli cell and identified 710 gene expression level change more than 2 times. Digital Gene Expression Tag Profiling analysis were performed using mRNA from control and Wt1-deficient Primary Sertoli cells, every group primare sertoli cell were from more than 30 mice.
Experiment type
RNA-seq of coding RNA 
Contacts
wang xiaona <xiaonayn@126.com>, Fei Gao, Xiaona Wang
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-46664.idf.txt
Sample and data relationshipE-GEOD-46664.sdrf.txt
Processed data (1)E-GEOD-46664.processed.1.zip
Additional data (1)E-GEOD-46664.additional.1.zip
Links