E-GEOD-46625 - Transcriptional profile of Escherichia coli K12 strain JM109 harboring pUCA1501

Status
Released on 15 October 2013, last updated on 22 October 2013
Organism
Escherichia coli
Samples (6)
Array (1)
Protocols (7)
Description
A1501 aroA is a gene derived from Pseudomonas stutzeri A1501, encoding a class II glyphosate-tolerant EPSP synthase. To understand the effect of class II EPSP synthase to E. coli under glyphosate shock, we constructed the class II EPSP synthase-expressing plasmid pUC-A1501. And pUC18 is the empty vector used as a control. RNA extracted from cells of E. coli K-12 JM109 cells with pUCA1501 till OD600 to achieve 0.4, or cells with pUC18 when their OD600 achieved 0.4.
Experiment type
transcription profiling by array 
Contacts
Liang Li <liliangene@163.com>, Min Lin, Wei Lu, Wujun Jin, Yusong Wan
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46625.idf.txt
Sample and data relationshipE-GEOD-46625.sdrf.txt
Raw data (1)E-GEOD-46625.raw.1.zip
Processed data (1)E-GEOD-46625.processed.1.zip
Array designA-AFFY-56.adf.txt
Links