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E-GEOD-46576 - The transcription factor Mef2 links the Drosophila core clock to Fas2, neuronal morphology and circadian behavior

Status
Released on 3 May 2013, last updated on 3 May 2014
Organism
Drosophila melanogaster
Samples (12)
Array (1)
Protocols (6)
Description
The transcription factor Mef2 regulates activity-dependent neuronal plasticity and morphology in mammals, and clock neurons are reported to experience activity-dependent circadian remodeling in Drosophila. We show here that Mef2 is required for this daily fasciculation-defasciculation cycle. Moreover, the master circadian transcription complex CLK/CYC directly regulates Mef2 transcription. ChIP-Chip analysis identified numerous Mef2 target genes implicated in neuronal plasticity, including the cell-adhesion gene Fas2. Genetic epistasis experiments support this transcriptional regulatory hierarchy, CLK/CYC->Mef2-> Fas2, indicate that it influences the circadian fasciculation cycle within pacemaker neurons and suggest that this cycle also contributes to circadian behavior. Mef2 therefore transmits clock information to machinery involved in neuronal remodeling, which contributes to locomotor activity rhythms. Mef2 ChIP-chip samples collected at 6 timepoints, input and IP samples
Experiment type
ChIP-chip by tiling array 
Contacts
Anna Sivachenko, Michael Rosbash
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46576.idf.txt
Sample and data relationshipE-GEOD-46576.sdrf.txt
Raw data (2)E-GEOD-46576.raw.1.zip, E-GEOD-46576.raw.2.zip
Array designA-AFFY-146.adf.txt
Links