E-GEOD-46527 - High-throughput sequencing for microRNAs in uninjured and regenerating newt hearts
Released on 24 September 2013, last updated on 25 November 2013
We have developed a standardized and reproducible heart resection/regeneration model system in the red-spotted newt. In order to ascertain the involvement of microRNAs in this amazing process, we made and sequenced cDNA libraries for microRNAs in order to provide sequence data that we could use in further quantitative and qualitative studies. We constructed small RNA cDNA libraries for uninjured (Uninj), 7dpi and 21dpi heart samples, which were sequenced using Illumina-Solexa technology at SciLifeLabs (Stockholm, Sweden).
RNA-seq of non coding RNA
Jamie Ian Morrison <email@example.com>, Jamie I Morrison, Nevin M Witman
miR-128 regulates non-myocyte hyperplasia, deposition of extracellular matrix and Islet1 expression during newt cardiac regeneration. Witman N, Heigwer J, Thaler B, Lui WO, Morrison JI. , Europe PMC 24055866