E-GEOD-46488 - Evolutionary conservation of the eumetazoan gene regulatory landscape

Released on 2 December 2013, last updated on 3 June 2014
Nematostella vectensis
Samples (29)
Protocols (2)
Bilaterian animals differ from other metazoans in their apparent bilateral symmetry and the development of a third germ layer. Both might have facilitated the evolution of the diverse and complex bilaterian body plans. The first cnidarian genome sequence revealed that despite their morphological simplicity, this sister group to all bilaterians shares an immense genomic complexity with vertebrates. This suggested that it might have been the complexity of gene regulation which increased during the evolution of bilaterians. We compared the gene regulatory landscape of cnidarians and bilaterians. To this end we generated the first genome-wide prediction of gene regulatory elements and profiled five epigenetic marks in a non-bilaterian animal, the cnidarian Nematostella vectensis. We found that the location of chromatin modifications relative to genes and distal enhancers is conserved among eumetazoans. Surprisingly, the genomic landscape of gene regulatory elements is highly similar between Nematostella and bilaterian model organisms. This suggests that complex regulation of developmental gene expression evolved in eumetazoans without a major increase in complexity in bilaterians. ChIP-seq of p300, RNA Pol2, and five histone modifications in Nematostella vectensis.
Experiment types
ChIP-seq, RNA-seq of coding RNA 
Alexandra Schauer, André F Rendeiro, Anna Gilles, Anna Schönauer, Carina Pribitzer, David Fredman, Eduard Renfer, Johannes Schinko, Michaela Schwaiger, Ulrich Technau
Exp. designProtocolsVariablesProcessedSeq. reads