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E-GEOD-4646 - Transcription profiling of human HUVEC cell line infected with Neisseria meningitidis wild type and mutants vs uninfected sampled at 3 time points reveals meningococcal adhesion suppresses proapoptotic gene expression and promotes expression of genes supporting early embryonic and cytoprotective signaling of human endothelial cells

Status
Submitted on 10 April 2006, released on 26 October 2007, last updated on 27 March 2012
Organism
Homo sapiens
Samples (23)
Array (1)
Protocols (19)
Description
Time courses of gene expression profiles of primary human umbilical vein endothelial cells (HUVEC) during infection with piliated and adherent wild-type (WT), frpC/frpA-deficient mutant, or the non-adherent (?pilD) Neisseria meningitidis MC58 bacteria defective in production of the type IV pilus, were analyzed respectively. Total RNA isolated from uninfected HUVEC (reference) and HUVEC after 1, 4 or 6 hours of infection with N. meningitidis mutants was labeled according to standard Affymetrix protocol and hybridized to HG-U133A GeneChips. Data were analyzed by Robust Multi-array Analysis algorithm. For every condition at least two biological replicates were analyzed, totally representing 23 Affymetrix GeneChips.
Experiment types
transcription profiling by array, co-expression, disease state, time series
Contact
Citation
Meningococcal adhesion suppresses proapoptotic gene expression and promotes expression of genes supporting early embryonic and cytoprotective signaling of human endothelial cells. Irena Linhartova, Marek Basler, Jeffrey Ichikawa, Vladimir Pelicic, Radim Osicka, Stephen Lory, Xavier Nassif, Peter Sebo. FEMS Microbiol Lett 263(1):109-18 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4646.idf.txt
Sample and data relationshipE-GEOD-4646.sdrf.txt
Raw data (1)E-GEOD-4646.raw.1.zip
Processed data (1)E-GEOD-4646.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-4646.eSet.r
Links