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E-GEOD-46455 - Comparison between wild type E. coli and adhE (alcohol dehydrogenase) mutants, grown under anaerobic condition

Status
Released on 26 September 2014, last updated on 5 October 2014
Organism
Escherichia coli BL21(DE3), Escherichia coli BW25113
Samples (4)
Array (1)
Protocols (7)
Description
To characterize anaerobic stress-induced expression in adhE mutants, the microarray experiment was performed with two serotypes of wild type E. coli and their adhE mutants: (1) K-12 strain, wild type; (2) B strain, wild type; (3) BW25113, adhE mutants; (4) BL21(DE3), adhE mutants. Under anaerobic growth condition in glucose-containing complex medium, the wild type strains 1 and 2 grew well whereas the mutant strains 3 and 4 experienced anaerobic stress and grew after 24 hours and 48 hours, respectively. For each of the four strains, RNA sampling was done after six hours of growth under anaerobic condition. Only one replicate was obtained for each strain.
Experiment type
transcription profiling by array 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46455.idf.txt
Sample and data relationshipE-GEOD-46455.sdrf.txt
Raw data (1)E-GEOD-46455.raw.1.zip
Processed data (1)E-GEOD-46455.processed.1.zip
Array designA-GEOD-13359.adf.txt
Links