Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-46455 - Comparison between wild type E. coli and adhE (alcohol dehydrogenase) mutants, grown under anaerobic condition
Released on 26 September 2014, last updated on 5 October 2014
Escherichia coli BL21(DE3), Escherichia coli BW25113
To characterize anaerobic stress-induced expression in adhE mutants, the microarray experiment was performed with two serotypes of wild type E. coli and their adhE mutants: (1) K-12 strain, wild type; (2) B strain, wild type; (3) BW25113, adhE mutants; (4) BL21(DE3), adhE mutants. Under anaerobic growth condition in glucose-containing complex medium, the wild type strains 1 and 2 grew well whereas the mutant strains 3 and 4 experienced anaerobic stress and grew after 24 hours and 48 hours, respectively. For each of the four strains, RNA sampling was done after six hours of growth under anaerobic condition. Only one replicate was obtained for each strain.
transcription profiling by array
Seungwoo Hwang <email@example.com>, Sang Joon Lee
Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase. Kim HJ, Jeong H, Hwang S, Lee MS, Lee YJ, Lee DW, Lee SJ. , PMID:25250024