Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-46390 - Expression data from peritoneal macrophages

Status
Released on 30 April 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (2)
Array (1)
Protocols (7)
Description
Colony Stimulating Factor 1(CSF1) is known to promote osteoclast progenitor survival but its role in regulating osteoclast differentiation and mature osteoclast function are less well understood. Macrophages have the potential to differentiate into osteoclasts and are also considered as osteoclast precursors. The microarray screen was designed to identify potential CSF1 targets in macrophage and osteoclast lineage. Wild type C57/BL6 mice were treated with 0.2 ml 10 % thioglycolate medium (FTG) / mouse by intra-peritoneal injection. Mice were sacrificed one week after injection followed by intra-peritoneal lavage with 15ml PBS. Macrophages were plated at a density of 1.5×10^6/well in 6-well plates and cultured in α-MEM supplemented with 10% FBS. At 80% confluence, cells were treated with 100ng/ml CSF1 for 12 hours and RNA extracted. Microarray analysis was performed using Affymatrix Mouse Genome 430 2.0 Arrays. Data were analyzed by Gene spring 6.2 software.
Experiment type
transcription profiling by array 
Contacts
chen yao <chen.yao@yale.edu>, Gang-qing Yao, Karl Insogna
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46390.idf.txt
Sample and data relationshipE-GEOD-46390.sdrf.txt
Raw data (1)E-GEOD-46390.raw.1.zip
Processed data (1)E-GEOD-46390.processed.1.zip
Array designA-AFFY-45.adf.txt
Links