E-GEOD-46135 - Epigenome analysis of DNA modification 5-hydroxymethylcytosine in normal, abnormal, and globozoospermia sperm genomes

Released on 18 April 2013, last updated on 12 June 2013
Homo sapiens
Samples (3)
Protocols (2)
Genome wide of 5-hydroxymethylcytosine profiling of normal and abnormal, and globozoospermia sperm genomes.Two semen samples were collected from a healthy man and a globozoospermia patient who had consulted a physician at ShengJing Hospital of China Medical University. Other semen samples obtained from two volunteers who were good health generally. 5-hmC enriched genomic DNA libraries were generated following the Illumina protocol for “Preparing Samples for CHIP sequencing of DNA”. 100bp single end sequencing on Illumina Hiseq2000 to get 5-hmC-enriched DNA fragment sequence was performed. Examination of 5hmC levels in normal, abnormal, and globozoospermia sperm genomes
Experiment type
methylation profiling by high throughput sequencing 
sun baofa <baofa1983@126.com>, Xiu-Xia Wang, You-Qi Liao, Yun-Gui Yang
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-46135.idf.txt
Sample and data relationshipE-GEOD-46135.sdrf.txt
Processed data (1)E-GEOD-46135.processed.1.zip