2 protocols
normalization data transformation protocol
basecall using illumina CASAVA v1.8 The 3' adaptor was removed from raw reads and reads with the length less than 10 were excluded to constitute the mappable reads. The mappable reads were aligned against human genome and miRbase using Bowtie. Genome_build: Homo sapiens 37.1; miRbase v17; Supplementary_files_format_and_content: comparison.xls: raw and normalized number of miRNA reads in each sample; statistical_analysis.xls: Statistical analysis for changed miRNAs in the various disease groups
nucleic acid library construction protocol
Trizol LS Illumina Truseq Small RNA Preparation