E-GEOD-4612 - Transcription profiling of MDR2 knockout mice vs heterozygotes sampled at multiple time points in the early stages of liver carcinogenesis to identify the molecular mechanisms of HCC initiation and promotion

Status
Submitted on 5 April 2006, released on 23 November 2007, last updated on 27 March 2012
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (3)
Description
We studied the molecular mechanisms of hepatocellular carcinoma (HCC) initiation and promotion using the Mdr2-knockout (Mdr2-KO) mice at pre-cancerous stages of liver disease. These mice lack the liver-specific P-glycoprotein responsible for phosphatidylcholine transport across the canalicular membrane. Portal inflammation ensues at an early age followed by the development of HCC between the ages of 12 and 15 months. Liver tissue samples of Mdr2-KO and control Mdr2-heterozygotes mice aged 3 and 12 months, were subjected to histological, biochemical and gene expression profiling analysis using Affymetrix Mouse Genome Array. The RNA samples from Mdr2-KO and control heterozygous mice aged 3 and 12M (3 males in each experimental group) were subjected to genome scale gene expression profiling with Affymetrix Mouse Array. The gene expression values were extracted with the help of MAS 5.0 software, and analyzed by cluster analysis, and by fold change filtering
Experiment types
transcription profiling by array, co-expression, individual genetic characteristics, time series
Contact
Citation
Multiple adaptive mechanisms to chronic liver disease revealed at early stages of liver carcinogenesis in the Mdr2-knockout mice. Mark Katzenellenbogen, Orit Pappo, Hila Barash, Naama Klopstock, Lina Mizrahi, Devorah Olam, Jasmine Jacob-Hirsch, Ninette Amariglio, Gidi Rechavi, Leslie Ann Mitchell, Ron Kohen, Eytan Domany, Eithan Galun, Daniel Goldenberg. Cancer Res 66(8):4001-10 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4612.idf.txt
Sample and data relationshipE-GEOD-4612.sdrf.txt
Raw data (1)E-GEOD-4612.raw.1.zip
Processed data (1)E-GEOD-4612.processed.1.zip
Array designA-AFFY-36.adf.txt
Links