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E-GEOD-46053 - Transcriptomic signature induced in bone marrow Mesenchymal Stromal Cells (MSC) after interaction with malignant multiple myeloma cells: implications in myeloma progression

Status
Released on 23 November 2014, last updated on 30 November 2014
Organism
Homo sapiens
Samples (37)
Array (1)
Protocols (7)
Description
Mesenchymal stromal cells (MSCs) derived from the BM of healthy donors (dMSCs) and myeloma patients (pMSCs) were co-cultured with the model myeloma cell line - MM.1S -, and the gene expression profile of MSCs induced by this interaction was analyzed using high density oligonucleotide microarrays. Deregulated genes in co-culture common to both d/pMSCs revealed functional involvement in tumor microenvironment cross-talk, myeloma growth induction and drug resistance, angiogenesis and signals for osteoclast activation and inhibition of osteoblasts. Additional genes induced by co-culture were exclusively deregulated in pMSCs and were predominantly associated to RNA processing, the ubiquitine-proteasome pathway, regulation of cell cycle and Wnt signaling. Primary MSCs from bone marrow (BM) samples of healthy donors (n=8) and multiple myeloma (MM) patients (n=13) were generated as described by Garayoa et al. 2009 (PMID:19357701). Ficoll-Paque density gradient separation medium was used to isolate mononuclear cells from BM aspirates. MSCs were selected by adherence to plastic and subsequently expanded until passage 2 when they were used for co-culture. For the MSC-MM.1S co-cultures we used a 6-well format transwell system with 1 µm pore size membrane. Briefly, 2 X 10^4 MSCs were first cultured attached to the lower side of the membrane, and when reached ≈ 85% confluence 1 X 10^6 MM.1S cells were seeded on the upper side. After 24 h co-culture in RPMI 1640 medium supplemented with 10% FBS and antibiotics, MSCs were recovered by trypsinization, washed once in PBS and stored at -80°C in RLT buffer (Qiagen) until RNA extraction. Absence of MM.1S cells in the recovered MSC population was assessed in selected samples by flow cytometry analysis.
Experiment type
transcription profiling by array 
Contacts
A Pandiella, Antonio Garcia-Gomez, E Diaz-Rodriguez, E M Ocio, F M Sanchez-Guijo, J C Montero, J F Blanco, J F San Miguel, Javier De Las Rivas, M Martin, Mercedes Garayoa
Citation
Transcriptomic profile induced in bone marrow mesenchymal stromal cells after interaction with multiple myeloma cells: implications in myeloma progression and myeloma bone disease. Garcia-Gomez A, De Las Rivas J, Ocio EM, D�az-Rodr�guez E, Montero JC, Mart�n M, Blanco JF, Sanchez-Guijo FM, Pandiella A, San Miguel JF, Garayoa M.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46053.idf.txt
Sample and data relationshipE-GEOD-46053.sdrf.txt
Raw data (1)E-GEOD-46053.raw.1.zip
Processed data (1)E-GEOD-46053.processed.1.zip
Array designA-GEOD-16100.adf.txt
Links