E-GEOD-4571 - Transcription profiling of human Treg cell versus their controls in human and mouse
Released on 13 June 2008, last updated on 27 March 2012
Homo sapiens, Mus musculus
We applied Affymetrix GeneChip technology for expression profiling of CD4+CD25+ (Treg) T cells versus their naive CD4+CD25- controls to obtain more information about the underlying molecular mechanisms involved in Treg specific immune suppression Experiment Overall Design: Expression profiles from: Experiment Overall Design:  human Treg cells obtained from peripheral blood by cell sorting were compared to their naive controls. RNAs were prepared from pools of ten individual healthy donors, labeled in two independend labeling reactions and hybridized onto two GeneChips of the same lot. Experiment Overall Design:  mouse Treg cells obtained from spleen by cell sorting of Balb/c mice were compared to their naive controls. RNAs were prepared from pools of three individual Balb/c mice, labeled in three independend labeling reactions and hybridized onto three GeneChips of the same lot. Experiment Overall Design: The differences in gene expression were used to unravel molecular mechanisms that help to explain the suppressive and anergic phenotypes of Treg cells after antigen activation.
transcription profiling by array, unknown experiment type
Signatures of human regulatory T cells: an encounter with old friends and new players. Susanne Pfoertner, Andreas Jeron, Michael Probst-Kepper, Carlos A Guzman, Wiebke Hansen, Astrid M Westendorf, Tanja Toepfer, Andres J Schrader, Anke Franzke, Jan Buer, Robert Geffers.